Biochemistry Sem - III

1. Discuss in detail the principal and working of pH meter. <9>
Or
Write different application of spectrophotometer in biochemistry. <9>
2. Discuss design and application of analytical ultracentrifuge. <9>
Or
Discuss in detail secondary structure of protein. <9>
3. Write any two. <12>
a. Optical isomers of carbohydrates.
b. Application of radioisotopes in biochemistry and medical science.
c. Structures and biological occurrences of disaccharides.
d. First law of thermodynamics and its importance.
4. Any five <20>
1. Write the importance of water in living organisms.
2. Draw structure and write importance of ATP.
3. Why monochromators are better wavelength selector over filters in spectrophotometers?
4. Explain working of photomultiplier tubes as photo detector.
5. Discuss the structure of starch and glycogen as well as their physiological importance.
6. Explain quaternary structure of protein.
7. Describe centrifugal force and relative centrifugal force.
5. Any five. <10>
1. Chiral carbon.
2. Peptide bonds.
3. Buffering capacity.
4. Reduction.
5. Absorption spectra.
6. Half-life of radio isotopes.
7. Primary structure of protein.
8. Density gradient centrifuge.
6. Any five. <10>
1. Which buffers are important in living system? Give example.
2. Discuss briefly entropy and enthalpy.
3. Write Beer-Lamberts laws of light absorption and their limitations.
4. Which one of the following has high energy and why? – Visible light or UV light.
5. Write the uses of Geiger counter and liquid scintillation counters.
6. Name the tests that are used for identification of reducing sugars.
7. What are aromatic amino acids? Give name and structure of any two aromatic amino acids.
8. Describe salting in and salting out of proteins.
7. Any five. <5>
1. What are the basic differences between weak and strong acid?
2. Give the name of different unit of radioactivity.
3. Why blue color filter is used to measure the absorption of a red colored solution by colorimeter?
4. Define molar extinction coefficient.
5. What do you understand by the term isotopes?
6. What are covalent bonds?
7. Give the structure of amino acid that lacks asymmetric carbon atom.