17 May, 2010

Biotechnology Paper - IX_2

1. What is radioactivity? Describe various types of radioactive decay.<15>
Or
Provide detail account of various technique used for immobilization of enzyme and cell.<15>
2. Describe any three.<15>
a. SDS-PAGE.
b. Density gradient centrifugation.
c. ISO electric focusing.
d. Agarsose gel electrophoresis.
3. Give the statement of Beer Lambert’s law. Describe instrumentation of UV visible spectroscopy.<15>
Or
a. Type of vibration in IR.<7>
b. Principle of NMR.<8>
4. Describe any three.<15>
a. GLC.
b. Principle of ion exchange chromatography.
c. ISO electric focusing.
d. TLC.
5. What is nanotechnology? Describe application of nanotechnology in various fields.<15>
Or
a. Characteristic of ideal biosensor.<7>
b. ECG.<8>

16 May, 2010

Biotechnology Sme - IV Final

1. Any three. <15>

a. Nitrogen cycle.

b. Factors affecting loss of biodiversity.

c. Types of positive interaction.

d. Conservation of biodiversity.

Or

What is ecosystem? Discuss different types of aquatic ecosystem with suitable example. <15>

2. Explain the metabolic diversity among prokaryotes. <15>

Or

Write short notes on: <15>

a. Bio magnification.

b. Xenobiotic.

c. Biodegradation.

3. Discuss in detail primary, secondary and tertiary treatment of waste water. <15>

Or

Write short notes on: <15>

a. Acid rain.

b. Bioremediation.

c. Physical properties of water.

4. What are bio fertilizers? Discuss its types and briefly explain advantages of bio fertilizers. <15>

Or

Discuss in detail about bioleaching. <15>

5. Define organic evolution. Discuss in detail natural selection of Darwin. <15>

Write short notes on: <15>

a. Prezygotic reproductive isolation mechanism.

b. Define allopatric, sympatric and quantum speciation.

c. Lamarkism or theory of inheritance of acquired character.

Biotechnology paper - V sem - V

1.
a. Explain various criteria that are considered for secondary screening of industrially important microorganism. <8>
b. Explain any two methods for long term preservation of important microorganism. <7>
Or
a. What are the applications of recombinant DNA technique in strain improvement? <8>
b. Explain the chemical mutagenesis and method for isolation of mutants. <7>
2.
a. Explain preparation of starter culture with example. <10>
b. Give advantages and disadvantages of continuous culture. <5>
Or
a. Enlist draw and explain the designs of various fermenter. <10>
b. Explain various devises for agitation of fermentation media <5>
3. Give detail account of media sterilization in industries. <15>
Or
Discuss in detail about row material in and various components of fermentation media. <15>
4.
a. Discuss the economically aspects of fermentation industry. <8>
b. Explain biological assay of fermentation production products <7>
Or
Explain in detail centrifugation and filtration as a separation method. <15>
5. Give detail account of fermentative production of penicillin. <15>
Or
Explain in detail about citric acid fermentation. <15>

Biotechnology Sem - V

1.
a. Explain various criteria that are considered for secondary screening of industrially microbes. <8>
b. Explain any two methods used for long term preservation of important microbes. <7>
Or
a. What are the applications of recombinant DNA technique in strain improvement? <8>
b. Explain chemical mutagenesis and methods for isolation of mutants. <7>
2.
a. Explain preparation of starter culture with example. <10>
b. Give advantages and disadvantages of continuous culture. <5>
Or
a. Enlist, draw and explain the designs of various fermenters. <10>
b. Explain various devises for agitation of fermentation media. <5>
3. Give detail account of media sterilization in industries. <15>
Or
Discuss in detail about raw materials and various components of fermentation media. <15>
4.
a. Discuss economic aspects of fermentation industry. <8>
b. Explain biological assay of fermentation products. <7>
Or
Explain in detail centrifugation and filtration as a separation method. <15>
5. Give detail account of fermentative product of penicillin. <15>
Or
Explain in detail about citric acid fermentation. <15>

Biotechnology Paper - IX_1

1. Explain the mechanism of immobilization of enzyme with its merits and demerits. <15>
Or
Describe various types of radiations, their uses and hazards associated with their uses. <15>
2.
a. Define centrifugation. Explain the basic principle of centrifugation in detail. <8>
b. Explain in the isoelectric focusing gel. <7>
Or
Explain in detail density and differential centrifugation. <15>
3. Define spectroscopy. State the Beer Lamberts law and derive it. Explain the reflection. <15>
Or
Write detail notes on atomic spectroscopy. <15>
4. Write short note on any two.
a. Affinity chromatography. <15>
b. Size exclusion chromatography.
c. Gas liquid chromatography.
d. Ion exchange chromatography.
Or
What is chromatography? Explain the basic principle of chromatography and effect of theoretical plate on efficiency of column. <15>
5. Write short note on any two. <15>
a. EEG.
b. ECG.
c. Deep brain stimulation.
d. Application of nanotechnology.
Or
What are biosensors? Explain its different type with examples. <15>

Biotechnology Paper - VIII sem - VI

1. Give a detail account the history of development of plant tissue culture.
Or
Define culture medium. Write in detail about medium components and its function in tissue culture medium.
2.
a. Write briefly the concept of totipotency.
b. What is protoplast? Explain different methods of protoplast fusion.
Or
a. Write the importance of anther and pollen culture.
b. What is somatic embryogenesis? Explain the principle and significant of it.
3. What is genetic transformation? Which are the methods utilized for the transfer of foreign gene into plant cell?
Or
a. Plant bodies.
b. Secondary metabolites.
c. Edible vaccine.
d. Application of tissue culture in agriculture.
4.
a. Discuss general procedure for dis-aggregation of tissues.
b. Give disadvantages of serum free media.
Or
a. Discuss disadvantages of serum in media.
b. Enlist general requirements of animal tissue culture laboratory and give lay out of the same.

Biotechnology Sem - II

1.
a. Explain properties of water. <8>
b. Write short note on buffer. <7>
Or
Write detail note on different chemical bonds. <15>
2. Explain in detail function and classification of carbohydrates. <15>
Or
a. Explain different four levels of protein structures. <7>
b. Write a short note on classification of amino acids. <8>
3. Explain in detail function and classification of lipids. <15>
Or
a. Write a note on semi conservative nature of DNA. <7>
b. Explain the transformation principle of Griffith. <8>
4. Answer any three <15>
a. Explain the block diagram of computer.
b. Structure of HTML with example of personal name and address.
c. Describe different applications of internet.
d. Give the names of any five input and output devices.
e. Draw the window and describe their parts.
5.
a. Explain the measures of dispersion and give their merits and demerits. <10>
b. Marks of 10 student are given calculate the standard deviation and draw your conclusion. <5>
1 2 3 4 5 6 7 8 9 10
45 50 60 55 70 30 40 50 40 60


Or
a. Write short note on “PROBABILITY”. <5>
b. Write a detail note on ANOVA. <10>

Biochemistry Sem - III

1. Discuss in detail the principal and working of pH meter. <9>
Or
Write different application of spectrophotometer in biochemistry. <9>
2. Discuss design and application of analytical ultracentrifuge. <9>
Or
Discuss in detail secondary structure of protein. <9>
3. Write any two. <12>
a. Optical isomers of carbohydrates.
b. Application of radioisotopes in biochemistry and medical science.
c. Structures and biological occurrences of disaccharides.
d. First law of thermodynamics and its importance.
4. Any five <20>
1. Write the importance of water in living organisms.
2. Draw structure and write importance of ATP.
3. Why monochromators are better wavelength selector over filters in spectrophotometers?
4. Explain working of photomultiplier tubes as photo detector.
5. Discuss the structure of starch and glycogen as well as their physiological importance.
6. Explain quaternary structure of protein.
7. Describe centrifugal force and relative centrifugal force.
5. Any five. <10>
1. Chiral carbon.
2. Peptide bonds.
3. Buffering capacity.
4. Reduction.
5. Absorption spectra.
6. Half-life of radio isotopes.
7. Primary structure of protein.
8. Density gradient centrifuge.
6. Any five. <10>
1. Which buffers are important in living system? Give example.
2. Discuss briefly entropy and enthalpy.
3. Write Beer-Lamberts laws of light absorption and their limitations.
4. Which one of the following has high energy and why? – Visible light or UV light.
5. Write the uses of Geiger counter and liquid scintillation counters.
6. Name the tests that are used for identification of reducing sugars.
7. What are aromatic amino acids? Give name and structure of any two aromatic amino acids.
8. Describe salting in and salting out of proteins.
7. Any five. <5>
1. What are the basic differences between weak and strong acid?
2. Give the name of different unit of radioactivity.
3. Why blue color filter is used to measure the absorption of a red colored solution by colorimeter?
4. Define molar extinction coefficient.
5. What do you understand by the term isotopes?
6. What are covalent bonds?
7. Give the structure of amino acid that lacks asymmetric carbon atom.

Biotechnology Sem - II Final

1.
a. Justify “Water is universal solvent”. <7.5>
b. Write note on ATP and other high energy molecule. <7.5>
Or
a. Explain various types of chemical bond involved in macromolecule with examples. <10>
b. State: “law of thermodynamics”. <5>
2. Define proteins. Explain structural levels of proteins. Write notes on biological importance of proteins. <15>
Or
a. Write nomenclature and classification of amino acids. <7.5>
b. Enlist the properties of monosaccharaides. <7.5>
3.
a. Explain in detail about semiconservative nature of DNA. <8>
b. What are vitamins? Explain different source and function of vitamins. <7>
Or
i. Chargaff’s rule. <3>
ii. Structure of nitrogen bases. <3>
iii. Function of lipid. <3>
4.
a. Write in detail about input and output devices of computer. <8>
b. What are the applications of Biocomputing? <7>
Or
ANSWER ANY FIVE. <15>
a. What is domain and webserver?
b. Write various heading tags of HTML.
c. How multimedia is useful for designing the webpage?
d. What are the applications of internet with reference to biotechnology?
e. What are PDF files? Write its uses.
f. Enlist uses of PowerPoint presentation.
5. What are measures of central tendency and dispersion? What are their merits and demerits? Calculate mean, median, mode, variance and standard deviation for following data: <15>

Protein unit/day (9): No. of families.
15-25 30
25-35 40
35-45 100
45-55 110
55-65 80
65-75 30
75-85 10


Or




a. Explain scattered diagram method. <5>
b. Calculate Karl Person’s coefficient of correlation from the following data. <5>

Gestational age (weeks): Crown-Heel length (cm)
Of new born:
34 46.8
30 47.0
32 47.0
28 46.2
35 47.0
37 47.0
40 47.4
29 46.6
38 47.8
c. what is student’s t-test? What is importance in analysis? <5>

Microbiology Sem - IV

1.
a. Answer following. <5>
1. What is hydrolysis?
2. Define atom.
3. What is valence shell?
4. Define buffer.
5. What is isotope?
b. Write essay on chemical bonds. <10>
Or
Write short notes on: <15>
a. Concept of pH and buffer.
b. Functional group of molecule.
c. Structure of atom.
2.
a. Answer following. <5>
1. How many asymmetric carbons are present in glucose?
2. Define racemic mixture.
3. Define tautomerization.
4. Name the amino acid which contain imidazole ring.
5. What do you mean by oligomeric protein?
b. Explain structure of protein. <10>
Or
Write short notes on: <15>
a. Hetero poly saccharides.
b. Mutarotation.
c. Properties of protein.
3.
a. Answer following. <5>
1. Write the statement of Erwin Chargaff’s rule.
2. Define PUFA.
3. Give full form of CPPP.
4. Define nucleosome.
5. Define waxes.
b. Explain different tests carried out to check the purity of fats and oils. <10>
Or
Write short notes on: <15>
a. Explain: DNA double helix.
b. Amphipathic lipids.
c. Properties of TAG.
4.
a. Answer following. <5>
1. What is chemoautotroph?
2. What is synchronous growth of bacteria?
3. Enlist the method used for quantitative measurement of bacterial growth.
4. What is selective media? Give any two examples of same.
5. What is chemo state?
b. Explain the nutritional requirement of bacteria. <10>
Or
Write short notes on: <15>
a. Nutritional types of bacteria.
b. Types of bacteria based on temperature and O2 requirement.
c. Different mode of reproduction of bacteria.
5.
a. Answer following. <5>
1. What is apoenzyme?
2. Define: isoenzyme.
3. Enlist the condition that affects enzyme activity.
4. What is EC no?
5. Enlist six different classes of enzyme.
b. Explain detail about regulation of enzyme activity. <10>
Or
Write short notes on: <15>
a. Reversible enzyme inhibition.
b. Irreversible enzyme inhibition.
c. Chemical and physical properties of enzyme.

15 May, 2010

Biology Sem - II

Botany <38>
1. Any five. <5>
a. Who discovered cell?
b. Who discovered nucleus?
c. How pH is express mathematically?
d. What do you mean by thermodynamics?
e. Define “karyotype”.
f. Write names of the parts of microscope.
g. Define photorespiration.
2. Any five. <25>
a. Describe Calvin cycle.
b. Explain history, methods and needs of Tissue culture.
c. Describe the process of protein synthesis.
d. Explain classification, nomenclature and action of enzyme.
e. Explain the ultra-structure of nucleus.
f. Explain chloroplast about its structure and function.
g. Describe the buffer solution and its operation.
3. Any four. <8>
a. How viroids are different from viruses?
b. What is β-oxidation?
c. Explain in brief: structure of histones.
d. Write a note: Maternal of chromosomes.
e. Explain working of microscope.
f. Differ: xanthophyll from carotene.
g. Give the laws of thermodynamics.



Zoology <37>
1. A. Any four. <4>
I. Classification of Eel up to class. (reason not necessary.)
II. Habit and habitat of amphioxus.
III. Cleavage pattern in amphioxus.
IV. Multiple allele theory for Rh.
V. Treatment of diabetes.
1. B. Any three. <9>
I. Write note on menopause.
II. Write note on fresh water aquarium.
III. What is median? Find the median from the following data.
: Life expectancy for two species of fishes in a lake.
Species ‘M’ Xi (mo): 34 36 37 39 40 41 42 43 79 (n=9)
Species ‘N’ Xi (mo): 34 36 37 39 40 41 42 43 44 45 (n-10)
IV. Explain class mammalian with two examples in detail.
1. C. Any one. <12>
I.
a. Embryogenesis and embryo development in amphioxus.
b. ABO blood group and its inheritance.
II.
a. Describe thalassemia in detail.
b. Poultry: kinds of flow and housing.
2.
A. Any four. <4>
I. Poultry apparatus.
II. Signs and symptoms of menopause.
III. Mode of bionomic.
IV. Diagnosis of diabetes.
V. Structure of amphioxus ova.
VI. Classification of human / parrot.
B. Any two. <8>
I. Write note on thalassemia. III. Explain sense organs in amphioxus.
II. Write a note on human lice. IV. General character of chordate.

Biotechnology-Immu. sem - V Paper - VII

1. Write detail note on cells of immune system. <15>
Or
What are lymphoid organs? Explain primary lymphoid organs in detail. <15>
2. What are antigens? Explain its characteristics and factors affecting immunogenicity. <15>
Or
Give detail account of antigen antibody reaction. <15>
3. Write in detail about antigen presenting cells of immune system. <15>
Or
a. Explain hybridoma technology for production of antibody. <10>
b. T-cell activation. <5>
4. What is cytokine? Explain properties and function of cytokine. <15>
Or
a. Activation and regulation of complement system. <8>
b. Vaccines. <7>
5. Explain auto immune diseases. <15>
Or
a. Explain immunological response to tuberculosis. <8>
b. Immunodeficiency diseases: AIDS. <7>