Biotechnology Paper - VIII sem - VI

1. Give a detail account the history of development of plant tissue culture.
Or
Define culture medium. Write in detail about medium components and its function in tissue culture medium.
2.
a. Write briefly the concept of totipotency.
b. What is protoplast? Explain different methods of protoplast fusion.
Or
a. Write the importance of anther and pollen culture.
b. What is somatic embryogenesis? Explain the principle and significant of it.
3. What is genetic transformation? Which are the methods utilized for the transfer of foreign gene into plant cell?
Or
a. Plant bodies.
b. Secondary metabolites.
c. Edible vaccine.
d. Application of tissue culture in agriculture.
4.
a. Discuss general procedure for dis-aggregation of tissues.
b. Give disadvantages of serum free media.
Or
a. Discuss disadvantages of serum in media.
b. Enlist general requirements of animal tissue culture laboratory and give lay out of the same.