Biotechnology Paper - IX_1

1. Explain the mechanism of immobilization of enzyme with its merits and demerits. <15>
Or
Describe various types of radiations, their uses and hazards associated with their uses. <15>
2.
a. Define centrifugation. Explain the basic principle of centrifugation in detail. <8>
b. Explain in the isoelectric focusing gel. <7>
Or
Explain in detail density and differential centrifugation. <15>
3. Define spectroscopy. State the Beer Lamberts law and derive it. Explain the reflection. <15>
Or
Write detail notes on atomic spectroscopy. <15>
4. Write short note on any two.
a. Affinity chromatography. <15>
b. Size exclusion chromatography.
c. Gas liquid chromatography.
d. Ion exchange chromatography.
Or
What is chromatography? Explain the basic principle of chromatography and effect of theoretical plate on efficiency of column. <15>
5. Write short note on any two. <15>
a. EEG.
b. ECG.
c. Deep brain stimulation.
d. Application of nanotechnology.
Or
What are biosensors? Explain its different type with examples. <15>