Biotechnology Sem - V

1.
a. Explain various criteria that are considered for secondary screening of industrially microbes. <8>
b. Explain any two methods used for long term preservation of important microbes. <7>
Or
a. What are the applications of recombinant DNA technique in strain improvement? <8>
b. Explain chemical mutagenesis and methods for isolation of mutants. <7>
2.
a. Explain preparation of starter culture with example. <10>
b. Give advantages and disadvantages of continuous culture. <5>
Or
a. Enlist, draw and explain the designs of various fermenters. <10>
b. Explain various devises for agitation of fermentation media. <5>
3. Give detail account of media sterilization in industries. <15>
Or
Discuss in detail about raw materials and various components of fermentation media. <15>
4.
a. Discuss economic aspects of fermentation industry. <8>
b. Explain biological assay of fermentation products. <7>
Or
Explain in detail centrifugation and filtration as a separation method. <15>
5. Give detail account of fermentative product of penicillin. <15>
Or
Explain in detail about citric acid fermentation. <15>